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Journal: Current biology : CB

Article Title: Genetic reprogramming of positional memory in a regenerating appendage

doi: 10.1016/j.cub.2019.10.038

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Digoxigenin-AP, Fab fragments antibody Roche Cat#11093274910; RRID:AB_2734716 Goat anti-rabbit Alexa Fluor 594 ThermoFisher Cat#A-11037, RRID:AB_2534095 p-Histone H3 Antibody Santa Cruz Cat#sc-8656; RRID:AB_2233067 Chemicals, Peptides, and Recombinant Proteins ARS Sigma A5533 BIO Sigma B1686-5MG Cas9 protein Tools Biotech TS-cas9-50ug EdU solution ThermoFisher Cat#A10044 Gadolinium Sigma G7532-5G Heat-inactivated Newborn Calf Serum ThermoFisher Cat#26010066 Horse serum ThermoFisher Cat#16050130 Hydroxyurea Sigma H8627 IWR-1 Sigma I0161-5G Tricaine Sigma A5040-25G Experimental Models: Organisms/Strains Branchiostoma floridae [ 52 ] N/A Monopylephorus sp N/A N/A Zebrafish: Ekkwill N/A N/A Zebrafish: fgf20a mutants [ 28 ] dob Zebrafish: lamb1a mutants [ 15 ] sde1 Zebrafish: plk2b deletion lines This paper as53 Zebrafish: pola2 deletion lines This paper as52 Zebrafish: pola2 mutants This paper pd311 Zebrafish: Tg(hsp70l:dnfgfr1-GFP) [ 25 ] pd1 Software and Algorithms FIJI [ 53 ] PMID: 22743772 LAS X Leica https://www.leica-microsystems.com/products/microscope-software/details/product/leica-las-x-ls/ Prism 7 software GraphPad https://www.graphpad.com Other HC PL APO 20x/0.75 CS2 Leica 15506517 Leica DFC7000 T Leica N/A Leica M205 Leica N/A Leica SP8 Confocal Leica N/A Open in a separate window KEY RESOURCES TABLE Genetic screen identifies a mutation ( mem , pola2 ) that disrupts fin regeneration pola2 inactivation causes a long-lasting reduction in size of new structures pola2 inactivation alters blastemal proliferation and size Progenitor cell cycle interference alters positional memory in several contexts

Techniques: Recombinant, Software